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PG TRB Bio Chemistry Study Material

PGTRB-BIO-CHEMISTRY UNIT II- Study Material-  -(English Medium)- Srimaan Coaching Centre-Trichy

PG TRB Bio Chemistry syllabus unit wise 

PG TRB Bio Chemistry syllabus  in Pdf

Unit I : Techniques in Bio-Chemistry Principles and application of light, phase contrast, Fluorescence, scanning and Transmission electron microscopy. Cytophotometry; Gel filtration; HPLC, Ultracentrifugation; X-ray diffraction; Fluorimetry; spectroscopy (UV, DRD/CD, visible, NMR, ESR, Atomic absorption and plasma emission); Principles and application of tracer techniques in biology, Liquid scientillation spectrometry.

 Unit II : Chemistry of Bio-Molecules Structure of carbohydrates, Polysaccharides, Glycoproteins, Peptidoglycans, cell wall polysaccharides; structure of aminoacides and proteins, forces involved, Ramacha.ndran Plot. Structure of Purine, Pyrimidine bases, Nucleotides, DNA, different types of RNA and vitamins.

 Unit III : Metabolosm Metabolosm of Carbohydrates, Aminoacids, lipids and Nucletic acids, Respiratory chain, oxidative phosphoerylation, free energy change; coupled reactions: Biological energy transducers; High energy compounds and group transfer potentials; Bio-energetics.

 Unit IV : Enzymes Enzyme kinetics; Regulation of enzyme activity Coenzymes, Activators / inhibitors, isoenzymes; Mechanism of enzyme action.

 Unit V : Clinical Bio-Chemistry Disorders of carbohydrate, Fat and Nitrogen, Metabolisms, Laboratory Dignosis, Liver and Kidney function tests; Blood coagulation disorders; Inborn errors of metabolism.

Unit VI : Bio - Membranes Structures and organization of membranes, hormones - structure, function and its role in signal transduction, Neurotranmitters; Transport across Membranes.

 Unit VII : Microbial Bio-Chemistry Classification of Micro-organisms - Viruses, bacteria, Fungi,Yeast; Basic principles of Bioprocess technology, Fermentation products - ethanol, glycerol, lacticacid, acetone, riboflavin, vitamin B12 and Pencillin, Preparation of media to culture microorganisms, Soil microbiology; use of micro organisms in seage treatment.

 Unit VIII : Molecular Biology Prokaryotic and Kukaryotic cell structure; Eukaryotic genome organisation; cell cycle, Replication, Transcription, Tranlation and Regulation of Gene Experssion; Lysogency and Lytic cycles in bacteriophages; bacterial transformation; principles of Geneticengineering Enzymes, vectors, C DNA and Genomic Libracy construction; Screening of Libraries; Western, Nothern and Southern blotting; Agarose Gel and SDS PAGE Electrophoresis; Dot Blot analysis; DNA sequencing methods; Autoradiography; Transgene Technology; PCR and its application, RFLP, RAPD; Molecular Pathogenesis of Cancer.

Unit IX : Immunology Antigens, Immunoglobulines, T and B Lymphocytes and their characterization; monpo-clonal antibodies; Accessory cells - Macrophages and Dendritic cells; Purification of immunologlobulins - Ion exchange and affinity chromate-graphy; Enzyme Linked Immunoabsorbant Assay, vaccines; Hypersensitivity reactions: Auto immunity, Antibody engineering; Antigen Presentation; Ratio immunoassay. Unit X : Tissue CulturePrimary cultures derived from plant, animal and human tissues, Maintenance of cell lines; Artifical Insemination technology; Callus culture; Sornaclonal Variation Micropropagation; somatic embrygenesis; protoplast fusion; artificial seeds.